FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n01_ALm205.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n01_ALm205.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17984529
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT491040.2730346733017028TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG70550.036.69956644-45
CGTATGC74850.035.09441846-47
TATGCCG75450.034.81567448-49
CTCGTAT77100.033.73581344-45
TATCTCG84300.030.40780840-41
TCTCGTA90050.028.51611142-43
ATCTCGT97350.026.62178442-43
GCTATCT105500.024.43270738-39
ATGCCGT109900.024.11822748-49
GTATGCC113400.023.24794846-47
GCCGTCT118250.022.21372850-51
AGCTATC118550.022.06378738-39
CCGTCTT121150.021.91720652-53
CTATCTC125200.020.83479540-41
TGCCGTC135250.019.9835450-51
AAGCTAT139550.018.82856236-37
CGTCTTC146550.018.21576152-53
GAAGCTA150850.017.93784536-37
AGGGGGG142100.016.6289874-75
AGTCACC183850.014.691977528-29
Produced by FastQC (version 0.11.5)