FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n01_ALm204.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n01_ALm204.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18680102
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT428190.2292225170933221TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG67350.035.05759444-45
TATGCCG70950.033.9454148-49
CGTATGC71950.033.63795546-47
CTCGTAT72800.032.79200444-45
TATCTCG78650.029.39036640-41
TCTCGTA85600.027.11218842-43
ATCTCGT94500.025.31287442-43
GCTATCT102950.022.68415838-39
ATGCCGT109800.021.8048848-49
GTATGCC111500.021.53587546-47
AGCTATC116300.020.69308738-39
CCGTCTT120000.020.68295152-53
GCCGTCT118000.020.55058350-51
CTATCTC125950.019.48466940-41
TGCCGTC137050.018.07530650-51
AAGCTAT142700.016.69839936-37
GAAGCTA148500.016.65413736-37
CGTCTTC149500.016.26807852-53
AGGGGGG145900.014.84512674-75
TACCGTA18150.013.6051539
Produced by FastQC (version 0.11.5)