FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n01_ALm203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n01_ALm203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17827201
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT435890.24450837795568695TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC67850.034.480746-47
TCGTATG66100.034.35466844-45
TATGCCG69550.033.4674348-49
CTCGTAT70450.032.80659544-45
TATCTCG78050.028.51988840-41
TCTCGTA83800.026.92894242-43
ATCTCGT92100.025.14691242-43
GCTATCT100400.022.38438838-39
ATGCCGT104050.022.3477648-49
GTATGCC106850.021.69526946-47
AGCTATC110550.021.08133538-39
CCGTCTT117100.020.16042752-53
GCCGTCT115050.020.08622250-51
CTATCTC120150.019.55481140-41
TGCCGTC132000.017.92080350-51
AAGCTAT134750.017.31302636-37
GAAGCTA144100.016.78317336-37
CGTCTTC141300.016.57314752-53
AGGGGGG141850.014.76700574-75
GAGCACA332650.014.3324279
Produced by FastQC (version 0.11.5)