FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n01_ALm202.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n01_ALm202.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17719859
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT427490.24124909797532812TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG66400.035.23637444-45
CGTATGC68500.034.95043646-47
TATGCCG70550.033.66544748-49
CTCGTAT70800.033.08009344-45
TATCTCG76900.029.59510240-41
TCTCGTA81900.028.10442242-43
ATCTCGT92750.025.12406242-43
GCTATCT99450.023.05220438-39
ATGCCGT104850.022.56172648-49
GTATGCC105450.022.47846246-47
CCGTCTT111800.021.71049352-53
GCCGTCT112950.021.00606550-51
AGCTATC116000.020.35721238-39
CTATCTC123900.019.2505540-41
TGCCGTC131450.018.4291450-51
AAGCTAT138950.017.14891236-37
CGTCTTC141150.017.02786452-53
GAAGCTA147950.016.71590836-37
AGGGGGG136200.015.60576574-75
AGTCACC177850.013.825376528-29
Produced by FastQC (version 0.11.5)