FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n01_ALm201.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n01_ALm201.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17899310
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT427790.23899803958923557TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG64700.035.06100544-45
CGTATGC67300.034.01991746-47
TATGCCG67300.033.84398748-49
CTCGTAT70500.031.94070444-45
TATCTCG76150.029.38820540-41
TCTCGTA82100.027.28417442-43
ATCTCGT90850.024.89168442-43
ATGCCGT100250.022.93342648-49
GCTATCT99150.022.64310538-39
GTATGCC105100.021.8973946-47
GCCGTCT108550.021.24501450-51
CCGTCTT111950.020.96061952-53
AGCTATC110950.020.32056638-39
CTATCTC119250.019.04546440-41
TGCCGTC127600.018.3896750-51
CGTCTTC136250.017.3094852-53
AAGCTAT138450.016.79914736-37
GAAGCTA146400.016.17899736-37
AGGGGGG136450.015.01999274-75
GAGCACA330900.014.3656269
Produced by FastQC (version 0.11.5)