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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2020-09-18, 04:09 based on data in: /beegfs/mk5636/logs/html/HFHMCBGXG/merged

        General Statistics

        Showing 16/16 rows and 4/5 columns.
        Sample Name% Dups% GCLengthM Seqs
        HFHMCBGXG_n01_ADT1_0909
        2.2%
        48%
        26
        18.2
        HFHMCBGXG_n01_GDO2_0909
        52.4%
        49%
        26
        65.0
        HFHMCBGXG_n01_HTO3_0909
        46.4%
        48%
        26
        21.7
        HFHMCBGXG_n01_cDNA1_0909
        7.5%
        49%
        26
        73.7
        HFHMCBGXG_n01_cDNA2_0909
        10.7%
        49%
        26
        130.7
        HFHMCBGXG_n01_cDNA3_0909
        11.2%
        49%
        26
        133.3
        HFHMCBGXG_n01_cDNA4_0909
        7.7%
        49%
        26
        80.6
        HFHMCBGXG_n01_undetermined
        63.5%
        45%
        26
        21.9
        HFHMCBGXG_n02_ADT1_0909
        2.1%
        43%
        57
        18.2
        HFHMCBGXG_n02_GDO2_0909
        67.4%
        46%
        57
        65.0
        HFHMCBGXG_n02_HTO3_0909
        42.9%
        43%
        57
        21.7
        HFHMCBGXG_n02_cDNA1_0909
        71.2%
        50%
        57
        73.7
        HFHMCBGXG_n02_cDNA2_0909
        73.3%
        50%
        57
        130.7
        HFHMCBGXG_n02_cDNA3_0909
        73.5%
        50%
        57
        133.3
        HFHMCBGXG_n02_cDNA4_0909
        71.8%
        50%
        57
        80.6
        HFHMCBGXG_n02_undetermined
        71.4%
        47%
        57
        21.9

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 8/8 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        21,912,478
        4.0
        cDNA1_0909
        73,657,944
        13.5
        cDNA2_0909
        130,701,004
        24.0
        cDNA3_0909
        133,263,495
        24.4
        cDNA4_0909
        80,593,334
        14.8
        ADT1_0909
        18,209,083
        3.3
        HTO3_0909
        21,739,948
        4.0
        GDO2_0909
        64,965,319
        11.9

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        GGGGGGGG
        16100962.0
        73.5
        GAAAAGAT
        170842.0
        0.8
        CTCTCTAA
        126926.0
        0.6
        CAACACAA
        119285.0
        0.5
        GAAAGGTA
        99758.0
        0.5
        CTCCTTAA
        93471.0
        0.4
        ATCAGATA
        90575.0
        0.4
        CTCCCTAA
        84236.0
        0.4
        TCCTCTAA
        81602.0
        0.4
        GAAGGGTA
        80652.0
        0.4
        TGTAAACA
        67451.0
        0.3
        CTCCACAA
        66528.0
        0.3
        TATAAAAA
        63398.0
        0.3
        GAAACGCT
        63204.0
        0.3
        AATCATAT
        58547.0
        0.3
        TGGTAACA
        57765.0
        0.3
        GATCAATA
        54544.0
        0.2
        GGGGGGGT
        54330.0
        0.2
        CCCTCTAA
        53808.0
        0.2
        GCCTTGTG
        53147.0
        0.2

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined% PhiX Aligned
        4.0
        616,232,560
        545,042,605
        4.0
        2.5

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (57bp , 26bp). See the General Statistics Table.

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

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        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        No samples found with any adapter contamination > 0.1%

        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.