FastQCFastQC Report
Fri 18 Sep 2020
HFHMCBGXG_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHMCBGXG_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21912478
Sequences flagged as poor quality0
Sequence length57
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11784735.378090967164918No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1332250.6079869195989609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCTC908150.050.4871563
CATAGCT912550.050.4245872
TAGCTCT907550.050.2595754
GCATAGC917650.050.1235581
GTTACCG63700.048.13655
ACGGAAC118600.047.8303641
TTAAACG95400.047.4180810
CCTAGTC70750.047.3595610
CGGAACA119900.047.3080832
TAGATCG78150.047.23142251
CTAGTCC70300.047.15499511
ACCTAGT71600.046.5469639
TAAACCG114750.046.42217311
TTAGAGT76450.046.4003033
TAATGCG37100.046.3743865
GAGATCG117350.045.86599751
ACCCGTC59550.045.681571
TAGTCCC72900.045.5431612
CACCTAG74300.044.9573148
TCACCTA75000.044.9083067
Produced by FastQC (version 0.11.5)