FastQCFastQC Report
Fri 18 Sep 2020
HFHMCBGXG_n02_cDNA4_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHMCBGXG_n02_cDNA4_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80593334
Sequences flagged as poor quality0
Sequence length57
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9707081.2044519711766732No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2314590.2871937274613804No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1369270.16989866680537127No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1184510.14697369387895032No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA1145420.14212341680764812No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG862580.10702870289495654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2610050.046.9164471
AGCAGTG2874950.042.5035322
CAGTGGT3571750.034.1397974
ATCAACG3655950.033.3472311
TGGTATC3665100.033.1897937
GTATCAA3686900.033.0402539
AGTGGTA3696150.033.0115745
TCAACGC3691700.032.99601712
TATCAAC3706750.032.94783810
CAACGCA3705750.032.90781813
AACGCAG3726900.032.76685314
GCAGTGG3734400.032.76233
GGTATCA3722150.032.6923648
ACGCAGA3732850.032.6915815
AGAGTAC3740200.032.49941319
GTGGTAT3785400.032.1773076
GCAGAGT3797500.032.06731417
GAGTACT3773450.032.02948820
CAGAGTA3808150.032.02726718
GTACTTT3768000.031.94099222