Basic Statistics
Measure | Value |
---|---|
Filename | HFHMCBGXG_n02_cDNA4_0909.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 80593334 |
Sequences flagged as poor quality | 0 |
Sequence length | 57 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 970708 | 1.2044519711766732 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 231459 | 0.2871937274613804 | No Hit |
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 136927 | 0.16989866680537127 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA | 118451 | 0.14697369387895032 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA | 114542 | 0.14212341680764812 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG | 86258 | 0.10702870289495654 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAGCAGT | 261005 | 0.0 | 46.916447 | 1 |
AGCAGTG | 287495 | 0.0 | 42.503532 | 2 |
CAGTGGT | 357175 | 0.0 | 34.139797 | 4 |
ATCAACG | 365595 | 0.0 | 33.34723 | 11 |
TGGTATC | 366510 | 0.0 | 33.189793 | 7 |
GTATCAA | 368690 | 0.0 | 33.040253 | 9 |
AGTGGTA | 369615 | 0.0 | 33.011574 | 5 |
TCAACGC | 369170 | 0.0 | 32.996017 | 12 |
TATCAAC | 370675 | 0.0 | 32.947838 | 10 |
CAACGCA | 370575 | 0.0 | 32.907818 | 13 |
AACGCAG | 372690 | 0.0 | 32.766853 | 14 |
GCAGTGG | 373440 | 0.0 | 32.7623 | 3 |
GGTATCA | 372215 | 0.0 | 32.692364 | 8 |
ACGCAGA | 373285 | 0.0 | 32.69158 | 15 |
AGAGTAC | 374020 | 0.0 | 32.499413 | 19 |
GTGGTAT | 378540 | 0.0 | 32.177307 | 6 |
GCAGAGT | 379750 | 0.0 | 32.067314 | 17 |
GAGTACT | 377345 | 0.0 | 32.029488 | 20 |
CAGAGTA | 380815 | 0.0 | 32.027267 | 18 |
GTACTTT | 376800 | 0.0 | 31.940992 | 22 |