Basic Statistics
Measure | Value |
---|---|
Filename | HFHMCBGXG_n02_cDNA3_0909.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 133263495 |
Sequences flagged as poor quality | 0 |
Sequence length | 57 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1535255 | 1.152044676601045 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 340946 | 0.2558435076312534 | No Hit |
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 217476 | 0.1631924781801648 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA | 189531 | 0.1422227444957826 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA | 184582 | 0.13850904930866476 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG | 137701 | 0.1033298728957994 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAGCAGT | 416160 | 0.0 | 46.829685 | 1 |
AGCAGTG | 460880 | 0.0 | 42.21428 | 2 |
CAGTGGT | 571985 | 0.0 | 33.92622 | 4 |
ATCAACG | 584055 | 0.0 | 33.25803 | 11 |
TGGTATC | 586140 | 0.0 | 33.034325 | 7 |
TCAACGC | 590130 | 0.0 | 32.89983 | 12 |
GTATCAA | 590205 | 0.0 | 32.87475 | 9 |
AGTGGTA | 591755 | 0.0 | 32.834755 | 5 |
TATCAAC | 592870 | 0.0 | 32.823112 | 10 |
CAACGCA | 592165 | 0.0 | 32.82112 | 13 |
AACGCAG | 595355 | 0.0 | 32.667713 | 14 |
GGTATCA | 595045 | 0.0 | 32.57945 | 8 |
ACGCAGA | 596655 | 0.0 | 32.577156 | 15 |
GCAGTGG | 600120 | 0.0 | 32.455013 | 3 |
AGAGTAC | 597635 | 0.0 | 32.420418 | 19 |
GTGGTAT | 605890 | 0.0 | 32.003605 | 6 |
GCAGAGT | 606840 | 0.0 | 31.973572 | 17 |
CAGAGTA | 608050 | 0.0 | 31.95851 | 18 |
GAGTACT | 602910 | 0.0 | 31.946815 | 20 |
CGCAGAG | 610225 | 0.0 | 31.85236 | 16 |