FastQCFastQC Report
Fri 18 Sep 2020
HFHMCBGXG_n02_cDNA3_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHMCBGXG_n02_cDNA3_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133263495
Sequences flagged as poor quality0
Sequence length57
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15352551.152044676601045No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3409460.2558435076312534No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2174760.1631924781801648No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1895310.1422227444957826No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA1845820.13850904930866476No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1377010.1033298728957994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4161600.046.8296851
AGCAGTG4608800.042.214282
CAGTGGT5719850.033.926224
ATCAACG5840550.033.2580311
TGGTATC5861400.033.0343257
TCAACGC5901300.032.8998312
GTATCAA5902050.032.874759
AGTGGTA5917550.032.8347555
TATCAAC5928700.032.82311210
CAACGCA5921650.032.8211213
AACGCAG5953550.032.66771314
GGTATCA5950450.032.579458
ACGCAGA5966550.032.57715615
GCAGTGG6001200.032.4550133
AGAGTAC5976350.032.42041819
GTGGTAT6058900.032.0036056
GCAGAGT6068400.031.97357217
CAGAGTA6080500.031.9585118
GAGTACT6029100.031.94681520
CGCAGAG6102250.031.8523616