Basic Statistics
Measure | Value |
---|---|
Filename | HFHMCBGXG_n02_cDNA1_0909.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 73657944 |
Sequences flagged as poor quality | 0 |
Sequence length | 57 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 851081 | 1.155450388351866 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 215309 | 0.292309272167575 | No Hit |
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 122218 | 0.1659264342214059 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA | 103317 | 0.14026592976855287 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA | 100334 | 0.13621612897585086 | No Hit |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG | 78222 | 0.10619628481620394 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAGCAGT | 230615 | 0.0 | 46.82987 | 1 |
AGCAGTG | 255360 | 0.0 | 42.246605 | 2 |
CAGTGGT | 317710 | 0.0 | 33.883747 | 4 |
ATCAACG | 324085 | 0.0 | 33.208027 | 11 |
TGGTATC | 324835 | 0.0 | 33.04906 | 7 |
TCAACGC | 327155 | 0.0 | 32.887142 | 12 |
GTATCAA | 327040 | 0.0 | 32.877518 | 9 |
CAACGCA | 328110 | 0.0 | 32.815052 | 13 |
TATCAAC | 328530 | 0.0 | 32.7966 | 10 |
AGTGGTA | 328800 | 0.0 | 32.770073 | 5 |
AACGCAG | 330070 | 0.0 | 32.658726 | 14 |
GGTATCA | 329925 | 0.0 | 32.579113 | 8 |
ACGCAGA | 330880 | 0.0 | 32.57032 | 15 |
GCAGTGG | 333655 | 0.0 | 32.436733 | 3 |
AGAGTAC | 331365 | 0.0 | 32.394688 | 19 |
GTGGTAT | 336280 | 0.0 | 31.974726 | 6 |
GCAGAGT | 337005 | 0.0 | 31.935755 | 17 |
CAGAGTA | 337690 | 0.0 | 31.918676 | 18 |
GAGTACT | 334450 | 0.0 | 31.895926 | 20 |
CGCAGAG | 339275 | 0.0 | 31.78609 | 16 |