FastQCFastQC Report
Fri 18 Sep 2020
HFHMCBGXG_n02_cDNA1_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHMCBGXG_n02_cDNA1_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73657944
Sequences flagged as poor quality0
Sequence length57
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8510811.155450388351866No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2153090.292309272167575No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1222180.1659264342214059No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1033170.14026592976855287No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA1003340.13621612897585086No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG782220.10619628481620394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2306150.046.829871
AGCAGTG2553600.042.2466052
CAGTGGT3177100.033.8837474
ATCAACG3240850.033.20802711
TGGTATC3248350.033.049067
TCAACGC3271550.032.88714212
GTATCAA3270400.032.8775189
CAACGCA3281100.032.81505213
TATCAAC3285300.032.796610
AGTGGTA3288000.032.7700735
AACGCAG3300700.032.65872614
GGTATCA3299250.032.5791138
ACGCAGA3308800.032.5703215
GCAGTGG3336550.032.4367333
AGAGTAC3313650.032.39468819
GTGGTAT3362800.031.9747266
GCAGAGT3370050.031.93575517
CAGAGTA3376900.031.91867618
GAGTACT3344500.031.89592620
CGCAGAG3392750.031.7860916