FastQCFastQC Report
Fri 18 Sep 2020
HFHMCBGXG_n02_HTO3_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHMCBGXG_n02_HTO3_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21739948
Sequences flagged as poor quality0
Sequence length57
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG672860.30950396017506576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAC4393250.050.679251
CATGTTA3951500.050.631542
TATCCCC4332350.050.62298
TACTATC4385300.050.6031955
TTACTAT4411150.050.599814
CTATCCC4344050.050.5888377
CAAGAAC6929450.050.57767
CCGTCCC3838100.050.567769
AGGACCA3836750.050.56031
TCGATAA2108400.050.5595861
ATCCCCC4307100.050.5572139
CTTACTA4410150.050.542733
TGTTACC3908150.050.5399554
CATCCAA3817400.050.5337266
CCATCCA3819450.050.528715
GCTTACT4399800.050.52562
ATGTTAC3956250.050.5244563
ACCGTCC3856800.050.5192958
TCCCCCA4305350.050.51484310
ACATGTT3964950.050.512431
Produced by FastQC (version 0.11.5)