FastQCFastQC Report
Fri 18 Sep 2020
HFHMCBGXG_n02_GDO2_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHMCBGXG_n02_GDO2_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64965319
Sequences flagged as poor quality0
Sequence length57
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3898450.6000817143682462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGCT55989800.050.9320642
ATAGCTC56004750.050.907483
TAGCTCT55982800.050.901144
GCTCTTA55906000.050.8935556
TCTTAAA55967550.050.887068
AGCTCTT55908500.050.8858225
CTCTTAA55945050.050.874787
CTTAAAC55913050.050.874299
TTAAACT22236300.050.8214910
TTAAACC14358000.050.78940610
TAAACTT13070800.050.7829811
TTAAACA14205800.050.7799610
CTTTTGC2447500.050.6979615
TTAAACG5348850.050.69194410
TAAACTC5487300.050.6675111
CGTCCCT1906750.050.6437074
TAAACCG6430150.050.64061711
ACTTTCT3535400.050.61520814
TTTCTAA1770200.050.5973216
TAAACCA4698650.050.5871611
Produced by FastQC (version 0.11.5)