FastQCFastQC Report
Fri 18 Sep 2020
HFHMCBGXG_n02_ADT1_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHMCBGXG_n02_ADT1_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18209083
Sequences flagged as poor quality0
Sequence length57
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG589660.323827399765271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC3123050.050.7761731
CCCGTCC3119450.050.765212
CGTCCCT3115950.050.764714
CCGTCCC3113250.050.756293
TTAGAGT4424050.050.670543
TAGAGTC4432700.050.666164
CGTAGTC2232050.050.6603552
CCCTAAG3141650.050.6328247
TCCCTAA3145100.050.6050536
TGCCACC4348650.050.6019511
CCTCATT1257200.050.5897374
GTCCCTA3143800.050.583495
AGAGTCT4435550.050.577125
TCTGCCA4364150.050.5759939
GCCACCC4332250.050.560712
CTGCCAC4360500.050.53856710
GAGTCTG4429700.050.5316056
AGTCTGC4408600.050.523787
CCACCCA4334350.050.51560613
GTCTGCC4382300.050.5075578
Produced by FastQC (version 0.11.5)