FastQCFastQC Report
Fri 18 Sep 2020
HFHMCBGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHMCBGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21912478
Sequences flagged as poor quality0
Sequence length26
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGG533650.2435370385768328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAG18800.017.394321
GGGACCT15450.016.8158531
ATGCGAT18750.016.7987141
CCGGGAT12900.016.7962271
GACCTGG14650.016.7755241
TGCGGGT16800.016.7184641
ATCGAGT19450.016.4004291
CGGAGCT19250.016.2060571
GACTGCG19000.016.0497261
CGATGGC23200.015.9113661
GCGCGAT10350.015.8946581
CTGAAGT21100.015.5933361
TCCCGAT18900.015.4977521
TAGTTGG15550.015.4820511
CGCTTCA20100.015.4708191
CCTCTGA23300.015.4556111
ACGAGCC18200.015.432431
CGATTGA18250.015.3901481
GGCCCTA13000.015.38119210
GATCGCG16250.015.3089711
Produced by FastQC (version 0.11.5)