FastQCFastQC Report
Fri 18 Sep 2020
HFHMCBGXG_n01_cDNA4_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHMCBGXG_n01_cDNA4_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80593334
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT198000.017.4331381
CAATGGA292500.017.4285018
ACTGAAC245850.017.307051
ATGCGAT212550.017.1244531
ATCGAGT241300.016.9370141
GACCTGG210600.016.7795771
GAATGAA217050.016.7233371
TGACGGC195350.016.6763151
CCGGGAT158450.016.6713941
ATGGGAG237150.016.6597981
CTGAAGT226150.016.5767251
AGCTTGA280450.016.5627521
GACTAAC217300.016.5475961
GGAAAGC195500.016.5049171
CGGACTG181450.016.4544411
TGCGGGT199550.016.4456211
GCGCGAT136450.016.441881
ACTGATG188700.016.4318161
GATGCTA209550.016.2383691
ATTGGTG242000.016.1853871
Produced by FastQC (version 0.11.5)