FastQCFastQC Report
Fri 18 Sep 2020
HFHMCBGXG_n01_cDNA3_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHMCBGXG_n01_cDNA3_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133263495
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC399200.017.4936921
CAATGGA482300.017.432548
ATGCGAT353950.017.0370961
GGGACCT334150.017.016931
GAATGAA357650.016.936351
GACCTGG370250.016.8975751
ATCGAGT406950.016.7918681
TGACGGC333750.016.779591
AGCTTGA460150.016.7502691
CGGACTG297050.016.67751
GACTAAC345050.016.6590921
ATGGGAG383950.016.5994341
GGAAAGC319750.016.456971
TGCGGGT326850.016.4299811
CCGGGAT260700.016.4208221
CTGAAGT386350.016.379821
ACTGATG308600.016.3579751
GCGCGAT219600.016.3377111
CGGAGCT363250.016.3145431
CTCGAAA294100.016.3040391
Produced by FastQC (version 0.11.5)