FastQCFastQC Report
Fri 18 Sep 2020
HFHMCBGXG_n01_cDNA2_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHMCBGXG_n01_cDNA2_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130701004
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA474700.017.4715048
ACTGAAC397350.017.2701441
GGGACCT328150.017.0655291
ATGCGAT351350.017.0431961
GAATGAA348550.017.0366291
ATCGAGT397700.017.008481
GACCTGG357700.016.8608741
TGACGGC329550.016.8139671
ACTGATG301400.016.6919271
GACTAAC339550.016.6752011
AGCTTGA457950.016.668711
CGGACTG294600.016.6188771
CTGAAGT380400.016.5777781
ATGGGAG388500.016.5539511
TGCGGGT321650.016.4992961
CCGGGAT253050.016.486011
CTCGAAA295050.016.4342061
GGAAAGC318350.016.2995971
TGCCAAA395400.016.2676031
GCGCGAT215300.016.2455081
Produced by FastQC (version 0.11.5)