FastQCFastQC Report
Fri 18 Sep 2020
HFHMCBGXG_n01_cDNA1_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHMCBGXG_n01_cDNA1_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73657944
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA264350.017.442188
ACTGAAC226000.017.2955111
ATGCGAT197200.017.0723971
GAATGAA192800.017.0003031
TGACGGC178500.016.9557881
ATCGAGT220950.016.9212551
GACCTGG202200.016.8926121
ATGGGAG217600.016.786461
AGCTTGA257050.016.7277341
CCGGGAT141600.016.7194611
GGGACCT175200.016.7099781
CGGACTG166600.016.7020361
GACTAAC191400.016.591631
CTGAAGT213550.016.561511
ACTGATG168250.016.472851
TGCGGGT186500.016.4108031
GCGCGAT125750.016.3929561
CTGCGGA158100.016.3220651
TCCCGAT220100.016.2913251
CGCTGGA161100.016.2850861
Produced by FastQC (version 0.11.5)