FastQCFastQC Report
Fri 18 Sep 2020
HFHMCBGXG_n01_HTO3_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHMCBGXG_n01_HTO3_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21739948
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAA102500.018.9566885
TCCCGTA105000.018.8196334
CTCCCGT114900.018.251223
AACTCCC142800.018.2466831
AAATGTG120300.018.23704710
GGCCCTA52600.018.11718610
ACTCCCG116300.018.0489642
GTAAATG133800.017.8319118
TAAATGT123450.017.8121669
CAATGGA59600.017.7510058
CATGGCG80200.017.610221
CGTAAAT121300.017.4024337
TTGACGA69300.017.40193710
ATAAGAG147150.017.2586541
ACTGAAC58450.017.1640931
GGGACCT43750.017.0326671
CCGTAAA119500.016.9877436
GAATGAA51900.016.9790341
TTGGGAC62950.016.949289
TGACGGC54250.016.9072911
Produced by FastQC (version 0.11.5)