FastQCFastQC Report
Fri 18 Sep 2020
HFHMCBGXG_n01_GDO2_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHMCBGXG_n01_GDO2_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64965319
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATGA274200.017.9066221
CTGACAC289000.017.7514324
ACTGAAC208100.017.6873931
TCTGACA306750.017.6794593
CTAATGG293700.017.6099741
CTCTGAC309750.017.5214122
CGGACTG203800.017.4716511
ATGGGAG225750.017.4609071
TTGTAGG257050.017.389251
AGCTTGA174700.017.3704381
TCTGGAA232350.017.3695661
GGCCCTA240700.017.29896510
ACTGATG162050.017.2882961
CCTCTGA423300.017.2631281
TGACGGC192600.017.262071
GTTGATT258100.017.2524538
CAATGGA194200.017.2188138
TGCGGGT218350.017.2098121
GATTCAG324900.017.1656861
GGATTAC229850.017.1233351
Produced by FastQC (version 0.11.5)