FastQCFastQC Report
Fri 18 Sep 2020
HFHMCBGXG_n01_ADT1_0909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHMCBGXG_n01_ADT1_0909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18209083
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT74550.018.1254771
ATGGCGC60800.018.042842
CACTGAG70100.017.8460062
CATGGCG89450.017.8011
TCTGGAA78850.017.7458881
CAATGGA55650.017.6634128
TTGTAGG88950.017.6536871
ACTGAAC60900.017.556671
TGGACCC53000.017.50897810
ACACTGA117050.017.4659081
ATGAATG57750.017.38482910
GTTGGAC53100.017.381758
TCTGACA69600.017.3420263
GGCCCTA36350.017.30353710
CCTCTGA96850.017.2360761
CTGACAC66750.017.2135544
GGGACCT36050.017.118341
TCGTCGC101300.017.107676
ATAAGAG97150.017.0798971
CTAGCCT102800.016.987631
Produced by FastQC (version 0.11.5)