FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34242391
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4408461.2874276215115936No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN565460.1651344965951706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAT130700.072.566433
CTATTTC232450.041.1746146
CGCTATT235100.040.5124054
AGCAGTG97400.030.3855172
TTTCTCT341450.028.5191339
GTATCAA127400.022.4240599
GCTATTT460300.021.2513315
CTATACT98550.017.92214
CCTATAC75000.017.8840923
GCCGCTA541300.017.5285112
GTATAGG69000.017.3684941
TATACTG83250.016.3366185
TACACTG64600.016.2119335
ATTTCTC637500.015.8246858
TACACCG51250.015.18429855
GGACCGT34600.014.4816066
GGGTACC137700.014.47201715-19
CTGGGTA141050.014.26620110-14
TAGACTG45850.013.9588025
ACTCGGA137750.013.95218175-79
Produced by FastQC (version 0.11.5)