FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC88396.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC88396.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9120112
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113100.12401163494483401No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG52700.017.1947085
TAGACTG36200.017.021815
TATACTG67350.016.8988635
CTATACT80300.016.7945754
CCTATAC51800.015.5390453
CTACCCT42400.015.2193384
TCTATAC72450.015.1136633
GTATAGA75150.014.9788131
CTACACT53750.014.8383564
GTATAGG53450.014.6740751
GTATAAG59400.014.4268081
CTACACC65900.014.0830324
TAGGACT50200.013.8656154
TATACCG36100.013.8559825
CTAGGAC40500.013.7869073
TCTACAC63300.013.7470243
GTCCAGG57100.013.7360651
GTATTGG72450.013.732731
GTAATAC55800.013.5154443
ATACTGT76250.013.5002416
Produced by FastQC (version 0.11.5)