FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC70635.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC70635.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6736152
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94450.1402135818787937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC41250.018.9866353
GTATAGG44350.017.5256561
TAGACTG30050.016.4041845
CTATACT63150.016.188514
TATACTG54900.015.449125
CCCTATA40650.014.8163072
GTATAGA60150.014.7335811
GTATTGG53900.014.69000151
TACACAG39550.014.6633685
GTATAAG46500.014.5282771
TACACTG40450.014.5163275
GTGTAGG35250.014.425241
TATACAG52050.014.205935
TAGGACA46000.014.0279234
CCAGGAC35700.014.0161623
ACAGCGT21200.013.6776398
TAATACT93500.013.6477864
CTAGACT34600.013.6206674
GGACCGT20450.013.4703016
CAGGACT30700.013.4616294
Produced by FastQC (version 0.11.5)