FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC56752.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC56752.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39366637
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG505780.12847935169062066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG250550.017.9385815
TAGACTG147550.017.146565
TATACCG143600.017.1133885
GTATAGG201500.017.049741
TACACTG191650.016.9835435
CTATACT272400.016.5023784
TACACCG234000.016.3262165
GTGTAGG206400.016.0819321
CTACACT189000.015.3447614
CCTATAC195000.015.1722253
TATACAG243500.015.1533835
CTACACC329500.014.9848934
ACTGTGC207650.014.9417338
GGACCGT150550.014.493596
TCTACAC246100.014.3496443
GTCCTAG146400.014.23877051
GTATAAG207900.014.1142741
GTATTGG256600.013.9264411
TGTACTG313350.013.8113275
TAGACAG148150.013.7987025
Produced by FastQC (version 0.11.5)