FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC56445.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC56445.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6307638
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82130.13020721861337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT57550.021.416814
GTATAGG49500.020.8415741
CCTATAC48050.019.4679323
CTACACT35050.017.9962674
TATACTG44800.017.9611515
GTAATAC35450.016.77343
TATACCG26050.016.696785
TATAGGG50450.016.6866152
TTATACT67450.016.3385244
CCCTATA46700.016.3171332
ACAGTCC21950.016.1825648
TACACTG33500.016.0131265
ACTACAC41850.015.9410113
TACACAG34400.015.5941795
GGACCGT21450.015.5460396
GTGTTAG33250.015.5136521
TAGACAG28650.015.43456655
GTGTAGG33900.015.4305051
CTACACC48000.015.4067164
TACACCG30850.015.0388295
Produced by FastQC (version 0.11.5)