FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC49051.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC49051.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29819818
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG343940.11533940280923244No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG207950.020.4683821
CCTATAC206750.019.6415183
CTATACT285400.019.3075924
TATACTG234950.018.2970035
TACACTG176850.017.5444375
TAGACTG131950.016.8117435
TACACAG172100.016.6386075
TACACCG140450.016.2072245
CTACACT183400.016.129814
TATACCG126400.016.0587485
GTGTAGG166500.015.7484481
GTATAGA236900.015.452911
GTATTGG241450.015.3422041
TATACAG236750.015.1877135
CTACACC208250.015.040674
GTATAAG201350.014.8263761
GTACAGG142200.014.2511351
GGACCGT99950.014.2158886
CCTACAC159250.014.2071743
ACAGTCC113650.014.160598
Produced by FastQC (version 0.11.5)