FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC44480.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC44480.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8171901
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104680.12809748918886807No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG69350.025.3441351
CTATACT86150.023.396634
CCTATAC70750.023.3696353
TATAGGG70600.020.2489662
TATACTG63800.020.1116315
GTATTGG69250.019.2977491
GTGTAGG43700.019.1128311
CCCTATA66600.019.0682
TACACTG48850.018.4014425
ACTGTGC51300.017.098588
TAGGACC37700.016.5394464
TACACCG39450.016.3545155
CCAGGAC50600.016.3379613
CTACACT48350.016.045424
TATTGGG75000.015.9648212
TATACTA131600.015.8095945
CTGTACT54650.015.7877634
GTACAGG39650.015.5698461
GTGTAGC56100.015.53556251
GTCTAGG32450.015.4434021
Produced by FastQC (version 0.11.5)