FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC44442.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC44442.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4850847
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60290.12428757287129444No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG30400.022.6539465
GTATAGG32150.020.3376581
TACACCG29700.020.0147975
CCTATAC32600.019.3529743
CTATACT37700.018.8481244
CTACACT24200.018.5763194
TACACTG22550.018.3240785
TATACCG19700.017.6631775
CTACACC38750.017.5889344
CCTACAC26350.017.0630683
GTGTAGG26800.016.5361521
CTAGGAC18450.016.5081713
ACTGTGC25250.016.3646728
TAGACTG17100.016.1094725
TAGACAG21100.015.8040795
GTGTATA34650.015.5155751
TTACACT29000.015.5016184
GTATTAC26300.015.46933651
CCCTATA30200.015.3791622
GTGTTAC19300.015.0571261
Produced by FastQC (version 0.11.5)