FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC37108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC37108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18366132
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG243280.1324612063117046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG146050.023.5728651
CTATACT186850.022.2733234
CCTATAC144500.022.1305683
TATACTG138550.019.8305645
TATAGGG148900.018.6634582
CCCTATA142200.018.0630762
TATACCG74600.017.9777495
TACACTG109000.016.7600945
TAGACTG83550.016.4857835
TATACTA286900.016.4747715
GGACCGT72000.016.4118046
GTGTAGG101100.016.0209871
GTATTGG144700.015.9622521
TACACCG88650.015.9462395
GTCTAGG72000.015.9389671
GTACTAG152800.015.6865041
TACACAG107600.015.5633155
CCTAGAC72700.015.5600873
GTATTAG125150.015.4958721
GTATTAC112350.015.3218371
Produced by FastQC (version 0.11.5)