FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC3401.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC3401.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25742242
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG305830.11880472571114822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT211000.017.5932924
GTATAGG144700.017.5196881
CCTATAC145750.017.3141173
TATACTG182700.017.2601175
TACACTG146100.016.9198765
TACACCG124050.015.8951685
CTACACC186400.015.5587154
TAGACTG104300.014.9433215
TACACAG137150.014.8526355
GTATTAG166450.014.7503521
CCTACAC134150.014.7030653
GTCTAGG82850.014.6417661
GTACAGG133750.014.5549431
TATACAG179200.014.4418635
TATACCG114050.014.4285785
CTACACT142000.014.3986044
GTATAGA196550.014.3393071
ACTGTGC152200.014.1936138
GTGTAGG128200.014.1651591
GGACCGT94100.014.0208376
Produced by FastQC (version 0.11.5)