FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC32315.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC32315.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8420
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110.13064133016627077No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACC100.00684391145.862271
CAAGATA100.0069679157144.994034
CTCCTTG100.0069679157144.994037
GATATAG100.0069679157144.994037
TATGCCA100.0069679157144.994034
ACCTTGT100.0069679157144.99403145
GGGGGGG2159.309522E-410.115864145
Produced by FastQC (version 0.11.5)