FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC32313.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC32313.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3578978
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41140.1149490161716557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGACT18150.017.9757444
GTATAGA26100.016.9791161
CCTATAC22300.016.907993
TATACTG29250.016.8528525
GTGTAGT24850.016.6638071
CCAGGAC19900.016.3965683
GTGTAGG20050.016.3051151
GTCCTAG16200.016.1440751
CTACCCT17550.016.1115934
GTATAGG21400.015.6159991
TGTACTG31250.015.54229455
TACACTG22700.015.3287155
CTATACT31450.015.2150824
TACTGTG22100.015.0888417
GTATTGG34050.014.935051
GTCTTAG13750.014.7938451
ATACTGT32850.014.7852876
CTAGGAC16000.014.5018533
GTCCAGG20050.014.4934361
GTAACAC17550.014.4605373
Produced by FastQC (version 0.11.5)