FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC32312.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC32312.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25817475
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG339680.13156979913798697No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT152300.016.3767364
TACACTG151600.016.3541035
TATACTG207350.016.0475395
GTATAGG157350.015.9709851
CCTATAC148350.015.8863883
TATACCG111100.015.7906895
CTATACT226700.015.223844
TACACAG145150.014.5336875
GTGTAGG136350.014.3823881
TACACCG113500.014.3071135
CTACCCT128400.014.28644854
AGTACTG194650.013.59371955
CTACACC175100.013.5817754
TATACAG191800.013.5689315
TAGACTG109400.013.4517425
CCTACAC140250.013.3397043
GTATAAG171650.013.1171721
GTATTAG183900.013.1122951
TCTATAC202700.013.0219793
GTATTGG218950.013.0035961
Produced by FastQC (version 0.11.5)