FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC32311.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC32311.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7372859
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94010.12750820272027444No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG46050.017.977321
CTATACT70850.016.7825534
CCTATAC40650.016.7678473
TACACCG33800.016.7293435
TACACTG44400.016.3274385
GGACCGT25950.016.2028586
CGTACAC29150.016.169063
TATACTG59150.015.687595
GTGTAGG42100.015.5242341
TAGACTG32950.015.4008115
CTACACT46850.015.3207354
CCTACAC40500.015.039533
TACACAG45100.014.9488365
ACACTGC40400.014.8935346
TCTATAC61050.014.7280753
CTACACC47250.014.4238124
CCTAGAC30800.014.3611463
TGGACCG45000.014.1765695
TATACAG57850.013.7844775
ACTGTGC44850.013.5773468
Produced by FastQC (version 0.11.5)