FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC32281.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC32281.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13697283
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180120.13150053189380698No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG77750.017.715245
TATACTG113900.016.4206315
TATACCG57950.016.0121775
TAGACTG62650.015.9680485
CCTACAC72150.015.77893453
GGACCGT43150.015.62402256
GTCTAGG53500.015.20511151
CTATACT126600.015.0622164
ATACCGT57500.015.0027136
CTACACT82650.014.9132694
GTCTTAG60100.014.62299251
GTATAGG82950.014.27238751
GTATTGG101100.014.2245781
GTATTAG101850.014.1198321
TACACCG60100.014.1125425
TACACAG79850.013.9810455
CTAGACT71750.013.7430714
TCTACAC88850.013.7109073
CTAGGAC62050.013.5559683
CCTATAC80950.013.5261573
Produced by FastQC (version 0.11.5)