FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC26633.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC26633.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21147164
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG276860.13092062841145036No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG171600.021.840895
GTATAGG135950.018.5386371
CTATACT190350.018.2446774
TACACTG126850.017.544665
CCTATAC128750.017.4030913
TACACCG104050.016.999845
CTACACT135700.016.5628624
CTACACC160450.016.448084
TACACAG120400.016.3771925
CCTACAC121250.016.3266123
TAGACTG93000.015.5899295
GTATAGA174400.015.5758831
TATACCG97350.015.4890375
TTATACT259550.015.2240084
GTGTAGG117250.015.0529291
TCTATAC171300.014.9428193
GTCTAGG88550.014.682231
ACTGTGC123100.014.6634218
GTATTGG182650.014.6337521
ACAGTCC72850.014.5283838
Produced by FastQC (version 0.11.5)