FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC26622.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC26622.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20056989
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG288410.14379526258901573No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG122300.018.172581
TAGACTG87300.016.4416625
TATACTG162750.016.3470695
CTATACT176500.016.2260254
CCTATAC115050.016.135463
TATACCG85100.016.0148585
TACACTG115800.015.9008365
TACACAG108750.015.5317895
TATACAG148550.015.4696835
CTACACT121000.014.5007264
GTGTAGG103950.014.3934351
GTCTAGG82550.014.3414211
GTATTGG156450.014.2987081
GTCCAGG118900.014.2329771
TTATACT246450.014.1506394
GTGTTAG109350.013.8819081
CCTAGAC79050.013.7599663
GTCCTGG111950.013.6892621
GTATAAG132650.013.5789481
TGTACTG177250.013.4965415
Produced by FastQC (version 0.11.5)