FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC26615.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC26615.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5969122
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67360.11284741709082172No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG29300.018.0615965
GTATAGG37300.017.5215971
GTGTAAG25150.017.3242051
TATACTG48400.017.0749875
CTATACT55700.016.9215454
TACACAG35250.016.0411825
CTACACT37750.015.5567874
TATACCG24600.015.3238945
TAGGACT32700.015.0769324
TAGACAG27550.014.9987175
GTCCTGG32600.014.9244131
CTACACC40650.014.8036694
CTAGACC21200.014.7056574
AGTACTG42700.014.6006255
GTAGGAC25100.014.4447493
GTGTACG24650.014.4350811
CTAGACT31250.014.3844594
CCTAGAC23250.014.3465863
TAGACTG24650.014.1164415
ACTGTGC35000.014.0843938
Produced by FastQC (version 0.11.5)