FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC26602.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC26602.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15048225
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG177240.11778133301435885No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC94550.017.486013
GTATAGG96450.016.9447231
CTATACT139600.016.7752724
TACACTG84400.016.577035
TATACTG113700.016.1944085
GTATAGA121000.014.9474861
GGACCGT47900.014.831366
TAGACAG67200.014.671065
TATACCG61250.014.4392815
TACACAG86900.014.4317225
TAGGACT83950.014.2500284
TGGACCG88100.014.1528655
TACACCG65650.014.1340655
TATAGGG99050.013.9927752
CTACACT87000.013.9171294
GTGTAGG72600.013.9069641
ACTGTGC84000.013.7217098
GTATTGG110450.013.6789211
TAGACTG68200.013.6055915
AGTACTG115650.013.6020995
Produced by FastQC (version 0.11.5)