FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC26596.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC26596.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20113562
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG233340.11601127637163422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG135900.021.590741
CCTATAC141000.021.5492733
CTATACT194350.019.9590574
TATACTG157200.017.9851365
TACACTG120700.016.6970275
TATAGGG136900.016.2718242
CCCTATA133400.015.9372412
CTACACT124250.015.93077854
TAGACTG87500.015.907245
GTATTGG162550.015.45947551
GTGTAGG103150.014.99741
TATACCG84100.014.9125445
TATACAG152950.014.7879095
GTATAGA167500.014.5690131
GTCCTAG89000.014.3624431
ATACTGT180400.014.3058986
GTCCTGG109050.014.2525891
GTATTAG142350.014.13279151
CTACACC140000.014.0867764
GTATTAC130250.013.94016551
Produced by FastQC (version 0.11.5)