FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC26595.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC26595.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40137474
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG475000.11834327192588154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG291650.019.9477961
CCTATAC282050.019.7192363
CTATACT386450.019.342984
TACACTG248050.017.7979555
TATACTG323850.017.7957085
TAGACTG180600.015.8551885
CTACACT254750.015.7671654
TACACCG187750.015.6761035
CCTACAC213900.015.4248673
GTATTGG331100.015.3993481
GGACCGT134200.015.3951476
GTATAGA332900.015.1415411
TACACAG239100.014.9775045
TATAGGG286400.014.972642
ACTGTGC231150.014.8653418
CTACACC279600.014.7029324
TATACAG309850.014.41190055
GTGTAGG219450.014.297911
CTTACAC171300.014.011723
TCTATAC317250.013.9884833
Produced by FastQC (version 0.11.5)