FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC26594.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC26594.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6707503
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88330.13168834959894912No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG42000.021.4439031
CTATACT61900.016.5155034
GTATTGG51250.016.0145971
CCAGGAC34300.015.644533
TAGACTG28200.015.1671765
CCTATAC43800.014.9002413
TACACAG35550.014.8862445
ACTGTCC27850.014.8370748
TATACTG49850.014.8332695
CTACACC45450.014.6763254
GTCCAGG39000.014.5265151
CTGTACT45550.014.484934
TACACTG38800.014.3866885
CAGGACC33950.014.3086414
CCTACAC33500.014.286443
GTGTAAG27500.014.2623961
TATACCG26600.014.1717525
ACTGCGC20550.014.11064158
GGACCGT21700.014.0309856
CTGCACT45650.013.976724
Produced by FastQC (version 0.11.5)