FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC26576.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC26576.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16271135
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG105200.019.1239931
TATACTG134450.018.4940915
TACACTG97300.018.2537845
CTATACT159350.017.063144
CCTATAC101800.016.5262133
TAGACTG71250.015.6687925
TAGACAG79450.015.3290435
CCTACAC87000.015.0866383
GGACCGT55250.014.9579116
CTACACT106250.014.8767254
CTACCCT79200.014.8309714
TACACAG100250.014.8241285
TATACAG138850.014.7232245
ATACTGT140750.014.1638946
GTGTAAG74900.014.1574671
GTCCTGG84850.014.0380421
GTATTGG130900.014.03768351
CTACACC109700.014.0122864
GTATTAC111000.013.937061
CCAGGAC87100.013.9037353
Produced by FastQC (version 0.11.5)