FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_IRGC19928.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_IRGC19928.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5716483
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62430.10921050583024564No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG61500.020.6662061
GTATAGG44650.019.681661
CCTATAC41650.019.499183
CTATACT52150.018.6299044
TATAGGG44850.017.6341652
TATACTG42500.017.398455
TGCGTTC50200.017.3289839
TACACAG32300.016.8328695
GTAGGAC22250.016.6208613
TCTATAC40400.016.5127643
TATTGGG65100.016.495732
GTCCTAG27900.016.3996351
TACACTG32100.016.2602375
GTGTAGG31650.016.0628341
GTGCGTT56150.015.750918
TATACCG25550.015.6052655
ATTGGGT71450.015.02013
GGACCGT23350.014.90218456
GGTGCGT59950.014.8734437
TACACCG30350.014.8092265
Produced by FastQC (version 0.11.5)