FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n02_GH4-1_Jae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n02_GH4-1_Jae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10214222
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161040.157662521922864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC85750.021.5640353
GTACTAC74550.020.420886
TAGTACT139300.019.3099374
TATACAG49700.019.253975
TATACCG24600.018.2708765
GTACTAG94200.018.0422741
ACTACTC89050.017.0954998
CAGCACT83000.016.8592134
AGTACTA138300.016.8261835
TACACTG41500.016.7697245
CCTAGTA96350.016.7185082
TACTACT93400.016.6873727
TACACAG36750.016.372825
CCAGCAC90550.016.1765863
CAATACT71750.016.1679944
GTATTAT73450.015.8217491
TAGGGCG43600.015.7957365
TACTAGG102850.015.5208192
GTATAGA55250.015.5122941
TATACTG39400.015.4555995
Produced by FastQC (version 0.11.5)