FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34242391
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1680130.4906579099572807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG64650.019.8463825
GTATAGG69900.018.84521
TATACTG80850.018.469875
CTATACT97800.017.0477164
CCTATAC73950.016.873893
TAGACTG46550.016.0396235
TACACCG53200.015.9422955
GTGTAGG60100.015.50011351
AGTACTG77050.014.6767055
CTACACC85800.014.5317664
TATACAG86100.014.2285355
TAGTACT150150.014.1938194
TACACAG63400.014.1778065
CTAGACT59500.013.8888134
GTACTAG88350.013.8389181
CTGCACT71900.013.8123934
TAGCACT70650.013.74896054
CTAGTAC90250.013.5047653
TATAGGG74900.013.4699162
GTATAGA85150.013.3333521
Produced by FastQC (version 0.11.5)