FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC88396.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC88396.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9120112
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG71200.018.1253645
TAGACAG42300.016.7970285
TATACCG36250.016.6003345
GTATAGG55400.016.2297211
GGACCGT33600.015.7518056
TACACTG50800.015.6991345
CTACACC62450.015.6728534
TAGACTG35900.015.3484985
TACACAG51500.015.2041895
GTGTAGG49700.014.7354751
GTCTAGG34600.014.6696941
CTATACT79850.014.6183284
CTACACT54600.014.6065224
GTATAAG58700.014.5761571
CCTAGAC31550.014.4773123
TACACCG45500.014.1816035
CCAGGAC54000.014.0975063
TATACAG69850.014.0124515
TGCACTG77750.013.80092055
GTGTAAG39150.013.7056321
Produced by FastQC (version 0.11.5)