FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC70635.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC70635.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6736152
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAG40500.018.4392365
GTATAGG40400.018.3069081
TACACTG38200.016.8922465
CCTATAC42350.016.4353353
GTATTGG56050.016.04141
TCTACAC42950.015.5304993
TATACTG54050.015.426345
TATACAG54700.015.3755775
TACACCG29250.015.3683255
CTACACT38700.015.3625624
TAGACAG30250.014.3809185
TAGACTG28800.014.349715
GTATAGA52600.014.3365221
CTACACC46100.014.3120294
CCTGTAC31500.014.2705883
CCAGTAC34750.014.1877943
CCTAGAC26100.014.1674083
GTATTAG47800.014.1075391
GTCTAGG26250.014.0876031
GCTACAC43850.014.0543283
Produced by FastQC (version 0.11.5)