FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC56752.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC56752.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39366637
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG229550.017.9079535
TATACTG246350.017.7167535
TACACTG193050.017.1627395
CTATACT274450.016.7481464
GTATAGG200450.016.711581
GTGTAGG199900.016.2497541
CCTATAC200400.015.5926623
TACACAG191350.015.3828845
TAGACTG148200.015.1653955
CTACACT195450.015.0602134
TAGACAG145900.014.8578565
CTACACC333250.014.7720374
TATACCG141350.014.7719165
GGACCGT151700.014.6721436
GTGTAGA247400.014.5073161
CCTACAC205500.014.2178523
TGCACCG367550.014.1430445
ACTGTGC204600.013.9955628
GTATAGA259700.013.9318941
GTATAAG206350.013.8795121
Produced by FastQC (version 0.11.5)